Gene Conversion of the Mating-Type Locus in SACCHAROMYCES CEREVISIAE

AUTOR(ES)

Klar, Amar J. S.

RESUMO

Tetrad analysis of MATa/MATα diploids of Saccharomyces cerevisiae generally yields 2 MATa:2MATα meiotic products. About 1 to 1.8% of the tetrads yield aberrant segregations for this marker. Described here are experiments that determine whether the aberrant meiotic segregations at the mating-type locus are ascribable to gene conversions or to MAT switches, that is, to mating-type interconversions. Diploid strains incapable of switching MATa to MATα, or the converse, nevertheless display changes of MATa to MATα, or the reverse. These events must be attributed to gene conversion. Further, we suggest that MATa and MATα alleles may represent nonhomologous sequences of DNA since they fail to display postmeiotic segregations.

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