Gene shuttling: moving of cloned DNA into and out of eukaryotic cells.
AUTOR(ES)
Lindenmaier, W
RESUMO
Successful shuttling of cloned DNA in eukaryotic cells should allow isolation of expressed genes. We tested the utility of cosmids for moving DNA into and out of eukaryotic cells. The unique cleavage of DNA at the cos site by the terminase function of lambda was exploited to maintain the linkage between the vector and inserted gene sequences, a prerequisite for successful rescue of the transforming DNA from high molecular weight DNA of the eukaryotic transformant. A cosmid recombinant containing the HSV thymidine kinase gene and a lambda recombinant containing the chicken thymidine kinase gene were used to test the feasability of this method. It was found that these recombinants can be rescued with high efficiency from DNA of HAT-resistant cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=320522Documentos Relacionados
- Cotransfer of circular and linear prokaryotic and eukaryotic DNA sequences into mouse cells.
- Nucleocytoplasmic shuttling: a novel in vivo property of antisense phosphorothioate oligodeoxynucleotides
- Role for topoisomerases in the release of DNA into the detergent-soluble fraction of eukaryotic cells.
- Expression of cloned immunoglobulin genes introduced into mouse L cells.
- Methylation of foreign DNA sequences in eukaryotic cells.
