GENE TRANSFER BY F′ STRAINS OF ESCHERICHIA COLI IV. : Effect of a Chromosomal Deletion on Chromosome Transfer
AUTOR(ES)
Pittard, James
RESUMO
Pittard, James (Yale University, New Haven, Conn.), and T. Ramakrishnan. Gene transfer by F′ strains of Escherichia coli. IV. The effect of a chromosomal deletion on chromosome transfer. J. Bacteriol. 88:367–373. 1964.—Evidence is presented that in an F′ strain of Escherichia coli. AB1206, the chromosomal region corresponding to the merogenote of F14 is deleted. AB1206 differs from all other F′ strains in its stability and in its relative inability to transfer chromosomal markers under conditions in which transfer of the merogenote occurs at a frequency of 100%. Its stability can be accounted for by the fact that the loss of the F merogenote, which contains approximately 10% of the genetic information of the cell, would be a lethal event. The failure of AB1206 to transfer chromosomal markers, except at very low frequencies, can be explained in terms of the crossover model proposed to explain chromosome transfer by F′ strains, since the homologous chromosomal region is missing in this strain. The conclusion that the chromosomal region is deleted is strengthened by the finding that the different enzymes coded for by the genes on F14 are present in AB1206 at the level found in haploid strains, but have double this activity in homozygous diploids carrying F14.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=277309Documentos Relacionados
- GENE TRANSFER BY F′ STRAINS OF ESCHERICHIA COLI K-12 I. : Delay in Initiation of Chromosome Transfer1
- GENE TRANSFER BY F′ STRAINS OF ESCHERICHIA COLI K-12 II. : Interaction Between F-Merogenote and Chromosome During Transfer1
- Chromosomal Integration of F′ Factors in Recombination-Deficient Hfr Strains of Escherichia coli
- Gene Transfer by F' Strains of ESCHERICHIA COLI K-12 III. an Analysis of the Recombination Events Occurring in the F' Male and in the Zygotes
- Mobilization of Haemophilus influenzae chromosomal markers by an Escherichia coli F' factor.