Gene Transfer in the Gastrointestinal Tract
AUTOR(ES)
Netherwood, Trudy
FONTE
American Society for Microbiology
RESUMO
The maximum in vivo transfer rate of plasmid pAMβ1 in the gut was 0.03 transconjugant per recipient cell, and this rate could be simulated in vitro only by forced filter mating. Transfer was not detected in liquid culture matings. Our findings demonstrate that in vitro methods, such as forced filter mating and liquid mating, underestimate the in vivo rates of gene transfer.
