General requirement for RNA polymerase II holoenzymes in vivo.
AUTOR(ES)
Thompson, C M
RESUMO
Yeast RNA polymerase II holoenzymes have been described that consist of RNA polymerase II, a subset of general transcription factors, and nine SRB regulatory proteins. The feature that distinguishes the RNA polymerase II holoenzymes from other forms of RNA polymerase II in the cell is their tight association with SRB proteins. We investigated the fraction of genes that require SRB proteins in vivo by examining the effect of temperature-sensitive mutations in SRB genes on transcription by RNA polymerase II. Upon transfer to the restrictive temperature, there is a rapid and general shutdown of mRNA synthesis in srb mutant cells. These data, combined with the observation that essentially all of the SRB protein in cells is tightly associated with RNA polymerase II molecules, argue that SRB-containing holoenzymes are the form of RNA polymerase II recruited to most promoters in the cell.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=41989Documentos Relacionados
- Requirement for 7-methylguanosine in translation of globin mRNA in vivo.
- Polyadenylylation of an mRNA precursor occurs independently of transcription by RNA polymerase II in vivo.
- Positionally exact initiation is required for the formation of a stable RNA polymerase II transcription complex in vivo.
- In Vivo Requirement of the RNA Polymerase II Elongation Factor Elongin A for Proper Gene Expression and Development
- Polyamine requirement for efficient translation of amber codons in vivo.