Generation of diverse high-affinity human monoclonal antibodies by repertoire cloning.
AUTOR(ES)
Persson, M A
RESUMO
Combinatorial libraries of antibody heavy and light chains derived from the peripheral blood lymphocytes of an individual immunized with tetanus toxoid have been expressed in Escherichia coli by using phage lambda vectors. Screening of the libraries allowed identification of a large number of human monoclonal Fab fragments specific for tetanus toxoid. Initial studies suggested considerable sequence diversity in these antibodies. The method should allow the generation of many human monoclonal antibodies of interest and the dissection of human humoral immune responses.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=51246Documentos Relacionados
- Immunodiagnosis of hepatitis B with high-affinity IgM monoclonal antibodies.
- High-affinity monoclonal antibodies for aflatoxins and their application to solid-phase immunoassays.
- High-affinity self-reactive human antibodies by design and selection: targeting the integrin ligand binding site.
- Regulation of High-Affinity Nitrate Transporter Genes and High-Affinity Nitrate Influx by Nitrogen Pools in Roots of Barley1
- High-affinity RNA ligands to human alpha-thrombin.