Genes activated in the presence of an immunoglobulin enhancer or promoter are negatively regulated by a T-lymphoma cell line.
AUTOR(ES)
Zaller, D M
RESUMO
The tissue-specific expression of immunoglobulin genes can be partially explained by a requirement for activating factors found only in B lymphocytes and their derivatives. However, loss of immunoglobulin expression upon fusion of an immunoglobulin-producing myeloma cell with a T lymphoma cell (BW5147) or fibroblast (L cell) suggests that negatively acting factors also play a role in the tissue specificity of immunoglobulin genes. Expression of a cloned immunoglobulin heavy-chain gene introduced into myeloma cells was suppressed after fusion of the myeloma transformants with BW5147. The presence of either the immunoglobulin heavy-chain enhancer or promoter conferred suppression, under similar conditions, upon a heterologous gene that is normally expressed in both B and T lymphocytes. These immunoglobulin heavy-chain gene control regions, or gene modifications induced by them, are subject to negative control by T-lymphocyte-derived factors.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=363371Documentos Relacionados
- Cell-type-specific receptors for alpha-fetoprotein in a mouse T-lymphoma cell line.
- Retroviral insertional activation of the c-myb proto-oncogene in a Marek's disease T-lymphoma cell line.
- Similarity between the interleukin 1 receptors on a murine T-lymphoma cell line and on a murine fibroblast cell line.
- Down-regulation of HLA antigens by the adenovirus type 2 E3/19K protein in a T-lymphoma cell line.
- Identification of the SL3-3 virus enhancer core as a T-lymphoma cell-specific element.