Genes that can be mutated to unmask hidden antigenic determinants in the cuticle of the nematode Caenorhabditis elegans.
AUTOR(ES)
Politz, S M
RESUMO
Rabbit antisera directed against a mixture of proteins solubilized from the wild-type adult Caenorhabditis elegans cuticle were used to isolate mutants, induced by ethyl methanesulfonate treatment, that exhibit alterations in surface antigenicity by immunofluorescence. Genetic mapping and complementation data for four such mutations define two genes, srf-2(I) and srf-3(IV). The mutant phenotypes observed by immunofluorescence appear to result from unmasking of antigenic determinants that are normally hidden in the wild-type cuticle. In support of this hypothesis, surface radioiodination experiments indicate that components labeled on the wild-type surface are missing or less readily labeled on the surface of srf-2 and srf-3 mutants.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=53801Documentos Relacionados
- Homeobox containing genes in the nematode Caenorhabditis elegans.
- Transposition of Tc1 in the nematode Caenorhabditis elegans.
- Transcription of class III genes in cell-free extracts from the nematode Caenorhabditis elegans.
- Normal and mutant thermotaxis in the nematode Caenorhabditis elegans.
- Cloning and analysis of three new homeobox genes from the nematode Caenorhabditis elegans.