Genetic analysis of Bacillus stearothermophilus by protoplast fusion.

AUTOR(ES)

Chen, Z F

RESUMO

Efficient and reliable protoplasting, regeneration, and fusion techniques were established for the prototrophic strain Bacillus stearothermophilus NUB36. Auxotrophic mutants were isolated, and protoplast fusion was used to construct isogenic mutant strains and for chromosomal mapping. Markers were mapped using two-, three-, and four-factor crosses. The order of the markers was hom-1-thr-1-his-1-(gly-1 or gly-2)-pur-1-pur-2. These markers may be analogous to hom, thrA, hisA, glyC, and purA markers on the Bacillus subtilis chromosome. No analogous pur-1 marker has been reported in B. subtilis. The relative order of three of the markers (hom-1-thr-1-gly-1) was independently confirmed by transduction.

Documentos Relacionados

• Genetic recombination in Micromonospora rosaria by protoplast fusion.
• Genetic recombination in Actinoplanes brasiliensis by protoplast fusion.
• Reconstitution of succinate dehydrogenase in Bacillus subtilis by protoplast fusion.
• Electron microscopic study of Bacillus subtilis protoplast fusion.
• Parasexual genetic analysis of Candida albicans by spheroplast fusion.