Genetic Analysis of Mecillinam-Resistant Mutants of Caulobacter crescentus Deficient in Stalk Biosynthesis

AUTOR(ES)

Seitz, Lisa C.

FONTE

American Society for Microbiology

RESUMO

Stalk synthesis in Caulobacter crescentus is a developmentally controlled and spatially restricted event that requires the synthesis of peptidoglycan at the stalk-cell body junction. We show that the β-lactam antibiotic mecillinam prevents stalk synthesis by inhibiting stalk elongation. In addition, mecillinam causes an increase in the diameter of the stalk at the stalk-cell body junction. We describe two mutations that confer resistance to mecillinam and that prevent stalk elongation. These mutations are probably allelic, and they map to a locus previously not associated with stalk synthesis.

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