Genetic Analysis of Mutations Affecting Growth of SACCHAROMYCES CEREVISIAE at Low Temperature
AUTOR(ES)
Singh, Arjun
RESUMO
A large number of genes control growth of the yeast Saccharomyces cerevisiae at low temperatures (< 10°). Approximately 47 percent of the mutants selected for inability to grow at 4–5°C show increased sensitivity to cycloheximide. In 3 of 4 cases tested, supersensitivity to cycloheximide and inability to grow at the low temperature segregate together and thus appear to be effects of the same mutation. Since many cold-sensitive mutants of bacteria have been found to have altered ribosomes and since cycloheximide resistance in yeast can be caused by ribosomal changes, this suggests that the mutants having low-temperature-sensitive growth may be defective in ribosome-assembly processes at the low temperatures. Two of the lts loci, lts1 and lts3 have been located on chromosome VII and another two, lts4 and lts10 on chromosome IV. A mutation, cyh10, conferring cycloheximide resistance, but not cold sensitivity, has been located close to the centromere on chromosome II.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1213157Documentos Relacionados
- Mutations Affecting Sexual Conjugation and Related Processes in SACCHAROMYCES CEREVISIAE. II. Genetic Analysis of Nonmating Mutants
- Mutations in cognate genes of Saccharomyces cerevisiae hsp70 result in reduced growth rates at low temperatures.
- “Killer Character” of Saccharomyces cerevisiae: Curing by Growth at Elevated Temperature
- Genetic Properties of Mutations at the PEP4 Locus in SACCHAROMYCES CEREVISIAE
- Sequence analysis of temperature-sensitive mutations in the Saccharomyces cerevisiae gene CDC28.
