Genetic Analysis of Repair of Ultraviolet Damage by Competent and Noncompetent Cells of Bacillus subtilis
AUTOR(ES)
Hadden, Charles T.
RESUMO
The repair of ultraviolet (UV) damage in Bacillus subtilis W23T− has been studied by transformation with deoxyribonucleic acid (DNA) extracted from irradiated cells before and after repair. The extent of repair of genetic markers by donor cells after low or moderate doses of UV was found to be related only to the initial degree of inactivation. After a very high dose, further inactivation occurred, also in proportion to initial damage. In addition, the competent recipient cells were shown to repair approximately 75% of the damage in transforming DNA. The sensitivities of markers irradiated either in vivo or in vitro appeared to be related to map position, the more proximal markers showing a greater resistance to UV inactivation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=251606Documentos Relacionados
- Repair of U.V. damages in Bacillus subtilis cultures competent for transformation: difference between competent and non-competent fractions.
- Different nuclease activities in competent and noncompetent Bacillus subtilis.
- DNA repair in competent cells of Bacillus subtilis.
- DNA repair in Bacillus subtilis: excision repair capacity of competent cells.
- Interactions between exogenous deoxyribonucleic acid and membrane vesicles isolated from competent and noncompetent Bacillus subtilis.