Genetic analysis of the human thymidine kinase gene promoter.
AUTOR(ES)
Kreidberg, J A
RESUMO
The promoter of the human thymidine kinase gene was defined by DNA sequence and genetic analyses. Mutant plasmids with deletions extending into the promoter region from both the 5' and 3' directions were constructed. The mutants were tested in a gene transfer system for the ability to transform TK- cells to the TK+ phenotype. This analysis delimited the functional promoter to within an 83-base-pair region upstream of the mRNA cap site. This region contains sequences common to other eucaryotic promoters including G X C-rich hexanucleotides, a CAAT box, and an A X T-rich region. The CAAT box is in an inverted orientation and is part of a 9-base-pair sequence repeated twice in the promoter region. Comparison of the genomic sequence with the cDNA sequence defined the first exon of the thymidine kinase gene.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=367858Documentos Relacionados
- Identification of a G1-S-phase-regulated region in the human thymidine kinase gene promoter.
- S-phase-specific regulation by deletion mutants of the human thymidine kinase promoter.
- Functional analysis of the human neurofilament light chain gene promoter.
- Analysis by cell-free transcription of the liver-specific pyruvate kinase gene promoter.
- Genetic analysis of the Mycobacterium smegmatis rpsL promoter.