Genetic characterization of the araE gene in Salmonella typhimurium lt2.
AUTOR(ES)
Lee, J H
RESUMO
Six L-arabinose transport-deficient mutants of Salmonella typhimurium LT2 were isolated on the basis of their inability to ferment low concentrations of L-arabinose. The mutations were localized between serA and lys on the S. typhimurium genetic map and assigned to the araE locus. An araE-lac fusion strain was constructed and used to determine that the direction of araE transcription was counterclockwise on the S. typhimurium genetic map. beta-Galactosidase activity was induced by L-arabinose in the araE-lac fusion strain, suggesting that araE expression is controlled at the level of transcription.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=217082Documentos Relacionados
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