Genome size of three Brazilian flies from the Sciaridae family

AUTOR(ES)

Saccuti, Cecília Ferreira, Soares, Maria Albertina de Miranda, Falco, José Ricardo Penteado, Fernandez, Maria Aparecida

FONTE

Genetics and Molecular Biology

DATA DE PUBLICAÇÃO

2005-12

RESUMO

We determined the genome size of the Brazilian sciarid flies Bradysia hygida, Rhynchosciara americana and Trichosia pubescens (Diptera: Sciaridae) using absorbance measurements of Feulgen-stained nuclei belonging to these species (and chicken erythrocytes as a standard) to calculate the amount of DNA in picograms (pg) and the number of base pairs (bp), or C-value, for each of these species. The C-values were: 3 x 10(8) bp (0.31 pg) for B. hygida; 3.6 x 10(8) bp (0.37 pg) for R. americana; and 1 x 10(9) bp (1.03 pg) for T. pubescens. The sciarids investigated in this work had considerably higher C-values than the average for previously described dipteran species, including D. melanogaster.

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