Genomic Analysis of the Nitrate Response Using a Nitrate Reductase-Null Mutant of Arabidopsis1[w]
AUTOR(ES)
Wang, Rongchen
FONTE
American Society of Plant Biologists
RESUMO
A nitrate reductase (NR)-null mutant of Arabidopsis was constructed that had a deletion of the major NR gene NIA2 and an insertion in the NIA1 NR gene. This mutant had no detectable NR activity and could not use nitrate as the sole nitrogen source. Starch mobilization was not induced by nitrate in this mutant but was induced by ammonium, indicating that nitrate was not the signal for this process. Microarray analysis of gene expression revealed that 595 genes responded to nitrate (5 mm nitrate for 2 h) in both wild-type and mutant plants. This group of genes was overrepresented most significantly in the functional categories of energy, metabolism, and glycolysis and gluconeogenesis. Because the nitrate response of these genes was NR independent, nitrate and not a downstream metabolite served as the signal. The microarray analysis also revealed that shoots can be as responsive to nitrate as roots, yet there was substantial organ specificity to the nitrate response.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=523318Documentos Relacionados
- A Genomic Analysis of the Shade Avoidance Response in Arabidopsis1[w]
- Ribonucleotide Reductase Regulation in Response to Genotoxic Stress in Arabidopsis1[W]
- A no hydrotropic response Root Mutant that Responds Positively to Gravitropism in Arabidopsis1[w]
- A Comprehensive Analysis of the NADP-Malic Enzyme Gene Family of Arabidopsis1[w]
- Functional Analysis of the RING-Type Ubiquitin Ligase Family of Arabidopsis1[w]
