Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor.
AUTOR(ES)
Kaushansky, K
RESUMO
Through the use of long single-sequence oligonucleotide probes, the complete gene for human granulocyte-macrophage colony-stimulating factor (hGM-CSF) has been cloned from a human genomic library. The gene is 2.5 kilobases in length, contains three introns, and is present as a single copy in the human genome. When subcloned into the mammalian expression vector pD3, the gene directs the synthesis of authentic hGM-CSF. In addition to its stimulation of in vitro granulopoiesis and monopoiesis, recombinant hGM-CSF stimulates in vitro erythropoiesis and megakaryopoiesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=323460Documentos Relacionados
- Expression cloning of a receptor for human granulocyte-macrophage colony-stimulating factor.
- Cloning, sequence, and expression of a human granulocyte/macrophage colony-stimulating factor.
- Regulation of human monocyte adherence by granulocyte-macrophage colony-stimulating factor.
- Specific human granulocyte-macrophage colony-stimulating factor antagonists.
- Interleukin 1 induces cultured human endothelial cell production of granulocyte-macrophage colony-stimulating factor.
