Germ-Line and Somatic Recombination Induced by in Vitro Modified P Elements in Drosophila Melanogaster
AUTOR(ES)
Sved, J. A.
RESUMO
The P element insertion Δ2-3(99B) has previously been shown to activate incomplete P elements elsewhere in the genome. We show that this element, in conjunction with a second incomplete P element, P[CaSpeR], also induces recombination in the male germ line. The recombination is induced preferentially in the region of the P[CaSpeR] element. Recombinant chromosomes contain the P[CaSpeR] element in more than 50% of cases, and alternative models of transposon replication and preferential chromosome breakage are put forward to explain this finding. As is the case with male recombination induced by P-M dysgenic crosses, recombination appears to be premeiotic in a high proportion of cases. The Δ2-3(99B) element is known to act in somatic cells. Correspondingly, we show that the Δ2-3(99B)-P[CaSpeR] combination elevates the incidence of somatic recombination.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1203925Documentos Relacionados
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