Global Gene Expression Profiles of Bacillus subtilis Grown under Anaerobic Conditions

AUTOR(ES)

Ye, Rick W.

FONTE

American Society for Microbiology

RESUMO

Bacillus subtilis can grow under anaerobic conditions, either with nitrate or nitrite as the electron acceptor or by fermentation. A DNA microarray containing 4,020 genes from this organism was constructed to explore anaerobic gene expression patterns on a genomic scale. When mRNA levels of aerobic and anaerobic cultures during exponential growth were compared, several hundred genes were observed to be induced or repressed under anaerobic conditions. These genes are involved in a variety of cell functions, including carbon metabolism, electron transport, iron uptake, antibiotic production, and stress response. Among the highly induced genes are not only those responsible for nitrate respiration and fermentation but also those of unknown function. Certain groups of genes were specifically regulated during anaerobic growth on nitrite, while others were primarily affected during fermentative growth, indicating a complex regulatory circuitry of anaerobic metabolism.

Documentos Relacionados

• Ultrastructure and respiratory capacity of Staphylococcus and Bacillus grown under aerobic and anaerobic conditions.
• Global Expression Profile of Bacillus subtilis Grown in the Presence of Sulfate or Methionine
• Expression profiles of sugarcane under drought conditions: Variation in gene regulation
• Suppression of Photoreactivating Enzyme Production in Escherichia coli Grown Under Anaerobic Conditions
• Dual Control of sbo-alb Operon Expression by the Spo0 and ResDE Systems of Signal Transduction under Anaerobic Conditions in Bacillus subtilis