Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells

AUTOR(ES)

Loonstra, Ate

FONTE

The National Academy of Sciences

RESUMO

The use of Cre/loxP recombination in mammalian cells has expanded rapidly. We describe here that Cre expression in cultured mammalian cells may result in a markedly reduced proliferation and that this effect is dependent on the endonuclease activity of Cre. Chromosome analysis after Cre expression revealed numerous chromosomal aberrations and an increased number of sister chromatid exchanges. Titration experiments in mouse embryo fibroblasts with a ligand-regulatable Cre-ERT show that toxicity is dependent on the level of Cre activity. Prolonged, low levels of Cre activity permit recombination without concomitant toxicity. This urges for a careful titration of Cre activity in conditional gene modification in mammalian cells.

Documentos Relacionados

• Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1.
• Recombination induced by triple-helix-targeted DNA damage in mammalian cells.
• Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase.
• Metformin (dimethyl-biguanide) induced DNA damage in mammalian cells
• CHROMOSOME DAMAGE INDUCED BY HYDROXYLAMINE IN MAMMALIAN CELLS*