Growth inhibition of Candida albicans by rabbit alveolar macrophages.

AUTOR(ES)

Peterson, E M

RESUMO

Normal rabbit alveolar macrophages were infected in vitro with Candida albicans. Early after infection, germ tube formation of phagocytized C. albicans was inhibited in contrast to extracellular (nonphagocytized) C. albicans. Over and 8-h period, plate counts of C. albicans incubated with alveolar macrophages revealed a decrease in colony-forming units in contrast to C. albicans alone. In addition, an assay was developed which specifically measured C. albicans [3H]leucine incorporation in the presence of alveolar macrophages. Using this assay, we observed a 71 to 93% inhibition of macromolecular synthesis in C. albicans when incubated with alveolar macrophages. Autoradiographic studies showed that the inhibition of leucine incorporation was restricted to the ingested Candida.

Documentos Relacionados

• Inhibition of specific amino acid uptake in Candida albicans by lysosomal extracts from rabbit alveolar macrophages.
• Fungicidal activity of rabbit alveolar and peritoneal macrophages against Candida albicans.
• Dissolution of metals by human and rabbit alveolar macrophages.
• In vitro phagocytosis of Candida albicans by peritoneal mouse macrophages.
• Toxicity of staphylococcal alpha toxin for rabbit alveolar macrophages.