Growth of Ribonucleic Acid Bacteriophage f2 in a Conditional Putrescine Auxotroph of Escherichia coli: Evidence for a Polyamine Role in Translation
AUTOR(ES)
Young, Delano V.
RESUMO
The ribonucleic acid (RNA) bacteriophage, f2, grows poorly in a conditional putrescine auxotroph during polyamine starvation. The addition of putrescine simultaneously with f2 enhances phage growth, shortens the latent period, and increases the burst size. The stimulation of f2 growth is reflected in higher rates of phage RNA and protein syntheses as measured by radioactive labeling of infected cells in the presence of rifampin. Putrescine does not affect f2 adsorption or the penetration of its RNA. Rather, in vitro assays demonstrate that in putrescine-supplemented cells more molecules of f2 replicase are made per incoming parental RNA than in polyamine-starved cultures. The ability of polyamines to stimulate the translation of a preformed messenger suggests a physiological role for these organic cations in normal protein synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=246611Documentos Relacionados
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