Growth rate paradox of Salmonella typhimurium within host macrophages.
AUTOR(ES)
Abshire, K Z
RESUMO
The growth rate of Salmonella typhimurium U937 within host macrophages was estimated by two independent methods. The extent to which ribosomal protein L12 is acetylated to produce ribosomal protein L7 changes markedly with the growth rate. By this measure, the intracellular bacteria appeared to be growing rapidly. Measurements of viable bacteria, however, indicated that the bacteria were growing slowly. A solution of this apparent growth rate paradox was sought by treating U937 cells infected with S. typhimurium X3306 with ampicillin or chloramphenicol to help determine the number of bacteria that were actively growing and dividing in the intracellular condition. Use of these antibiotics showed that by 2 h after invasion, the intracellular bacteria consisted of at least two populations, one static and the other rapidly dividing. This finding implies that previously described changes in the gene expression of S. typhimurium are important for the survival and/or multiplication of the bacteria within the macrophage.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=204790Documentos Relacionados
- Salmonella typhimurium loci involved in survival within macrophages.
- Flagella help Salmonella typhimurium survive within murine macrophages.
- Acidification of phagosomes containing Salmonella typhimurium in murine macrophages.
- Salmonella typhimurium invasion induces apoptosis in infected macrophages.
- Analysis of proteins synthesized by Salmonella typhimurium during growth within a host macrophage.
