Guidance in vitro of the pollen tube to the naked embryo sac of torenia fournieri

AUTOR(ES)

Higashiyama, T

RESUMO

The precise guidance of the pollen tube to the embryo sac is critical to the successful sexual reproduction of flowering plants. We demonstrate here the guidance of the pollen tube to the embryo sac in vitro by using the naked embryo sac of Torenia fournieri, which protrudes from the micropyle of the ovule. We developed a medium for culture of both the ovule and the pollen tube of T. fournieri and cocultivated them in a thin layer of solid medium. Although pollen tubes that had germinated in vitro passed naked embryo sacs, some pollen tubes that grew semi-in vitro through a cut style arrived precisely at the site of entry into the embryo sac, namely, the filiform apparatus of the synergids. When pollen tubes were unable to enter the embryo sac, they continuously grew toward the same filiform apparatus, forming narrow coils. Pollen tubes selectively arrived at complete, unfertilized embryo sacs but did not arrive at those of heat-treated ovules or those with disrupted synergids. These results convincingly demonstrate that pollen tubes are specifically attracted to the region of the filiform apparatus of living synergids in vitro.

Documentos Relacionados

• Blazing New Trails (Pollen Tube Guidance in Flowering Plants).
• Inter-Tissue Correlations in Organ Fragments: Organogenetic Capacity of Tissues Excised from Stem Segments of Torenia fournieri Lind Cultured Separately in Vitro
• In Vitro Measurement of Pollen Tube Growth Inhibition
• Genetic Evidence for a Long-Range Activity That Directs Pollen Tube Guidance in Arabidopsis.
• Directional Guidance of Nicotiana alata Pollen Tubes in Vitro and on the Stigma1