H-NS Is a Repressor of the Proteus mirabilis Urease Transcriptional Activator Gene ureR
AUTOR(ES)
Coker, Christopher
FONTE
American Society for Microbiology
RESUMO
Expression of Proteus mirabilis urease is governed by UreR, an AraC-like positive transcriptional activator. A poly(A) tract nucleotide sequence, consisting of A6TA2CA2TGGTA5GA6TGA5, is located 16 bp upstream of the ς70-like ureR promoter P2. Since poly(A) tracts of DNA serve as binding sites for the gene repressor histone-like nucleoid structuring protein (H-NS), we measured β-galactosidase activity of wild-type Escherichia coli MC4100 (H-NS+) and its isogenic derivative ATM121 (hns::Tn10) (H-NS−) harboring a ureR-lacZ operon fusion plasmid (pLC9801). β-Galactosidase activity in the H-NS− host strain was constitutive and sevenfold greater (P < 0.0001) than that in the H-NS+ host. A recombinant plasmid containing cloned P. mirabilis hns was able to complement and restore repression of the ureR promoter in the H-NS− host when provided in trans. Deletion of the poly(A) tract nucleotide sequence from pLC9801 resulted in an increase in β-galactosidase activity in the H-NS+ host to nearly the same levels as that observed for wild-type pLC9801 harbored by the H-NS− host. Urease activity in strains harboring the recombinant plasmid pMID1010 (encoding the entire urease gene cluster of P. mirabilis) was equivalent in both the H-NS− background and the H-NS+ background in the presence of urea but was eightfold greater (P = 0.0001) in the H-NS− background in the absence of urea. We conclude that H-NS represses ureR expression in the absence of urea induction.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=111335Documentos Relacionados
- Proteus mirabilis urease: transcriptional regulation by UreR.
- UreR, the Transcriptional Activator of the Proteus mirabilis Urease Gene Cluster, Is Required for Urease Activity and Virulence in Experimental Urinary Tract Infections
- Identification of the Domains of UreR, an AraC-Like Transcriptional Regulator of the Urease Gene Cluster in Proteus mirabilis
- The Escherichia coli nucleoid protein H-NS functions directly as a transcriptional repressor.
- The histone-like protein H-NS acts as a transcriptional repressor for expression of the anaerobic and growth phase activator AppY of Escherichia coli.