Heat mutagenesis in bacteriophage T4: the transition pathway.

AUTOR(ES)

Baltz, R H

RESUMO

G-C leads to A-T transitions are induced by heat, and arise from the deamination of cytosine (5-hydroxymethylcytosine in the case of bacteriophage T4) generating uracil. The reaction is proton-catalyzed, and is also characteristic of acid mutagenesis. Mutation rates and activation energies of mutation are site-specific, and are presumably influenced by neighboring bases. Rates of heat-induced mutation in bacteriophage T4 under conditions of temperature, pH, and ionic strength similar to those prevailing in higher eukaryotic cells suggest that heat mutagenesis may present a serious challenge to organisms with large genomes, and may comprise an important determinant of the rates of spontaneous mutation.

Documentos Relacionados

• Heat mutagenesis in bacteriophage T4: the transversion pathway.
• Heat mutagenesis in bacteriophage T4: another walk down the transversion pathway.
• Mechanics of Frameshift Mutagenesis in Bacteriophage T4: Role of Chromosome Tips*
• Prohead core of bacteriophage T4 can act as an intermediate in the T4 head assembly pathway.
• Thymineless Mutagenesis in Bacteriophage T4