Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix.
AUTOR(ES)
Enat, R
RESUMO
The culture conditions found to result in stable proliferation of normal rat hepatocytes are: (i) subconfluent cell densities; (ii) serum-free medium; (iii) hormonally defined medium containing epidermal growth factor, insulin, glucagon, prolactin, and other growth factors; and (iv) substrata of liver extracellular matrix depleted of growth inhibitors. Serum was found deleterious to parenchymal cells: it was inhibitory to the expression of liver-specific functions, cytostatic to parenchymal cells at all seeding densities, and cytotoxic to them at low seeding densities. These studies emphasize the relevance of synergies in the influences of hormones and extracellular matrix in regulating hepatocellular physiology.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=344845Documentos Relacionados
- Differential response to growth factor by rat mammary epithelium plated on different collagen substrata in serum-free medium.
- Production of human interferon-gamma in serum-free medium.
- Growth of embryonal carcinoma cells in serum-free medium.
- Fibrinogen synthesis in serum-free hepatocyte cultures: Stimulation by glucocorticoids
- Growth in serum-free medium improves isolation of Chlamydia pneumoniae.