Heritable endogenous gene regulation in plants with designed polydactyl zinc finger transcription factors

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The National Academy of Sciences

RESUMO

Zinc finger transcription factors (TFsZF) were designed and applied to transgene and endogenous gene regulation in stably transformed plants. The target of the TFsZF is the Arabidopsis gene APETALA3 (AP3), which encodes a transcription factor that determines floral organ identity. A zinc finger protein (ZFP) was designed to specifically bind to a region upstream of AP3. AP3 transcription was induced by transformation of leaf protoplasts with a transformation vector that expressed a TFZF consisting of the ZFP fused to the tetrameric repeat of herpes simplex VP16's minimal activation domain. Histochemical staining of β-glucuronidase (GUS) activity in transgenic AP3∷GUS reporter plants expressing GUS under control of the AP3 promoter was increased dramatically in petals when the AP3-specific TFZF activator was cointroduced. TFZF-amplified GUS expression signals were also evident in sepal tissues of these double-transgenic plants. Floral phenotype changes indicative of endogenous AP3 factor coactivation were also observed. The same AP3-specific ZFPAP3 was also fused to a human transcriptional repression domain, the mSIN3 interaction domain, and introduced into either AP3∷GUS-expressing plants or wild-type Arabidopsis plants. Dramatic repression of endogenous AP3 expression in floral tissue resulted when a constitutive promoter was used to drive the expression of this TFZF. These plants were also sterile. When a floral tissue-specific promoter from APETALA1 (AP1) gene was used, floral phenotype changes were also observed, but in contrast the plants were fertile. Our results demonstrate that artificial transcriptional factors based on synthetic zinc finger proteins are capable of stable and specific regulation of endogenous genes through multiple generations in multicellular organisms.

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