Heterochromatic Position Effect at the Rosy Locus of DROSOPHILA MELANOGASTER: Cytological, Genetic and Biochemical Characterization
AUTOR(ES)
Rushlow, C. A.
RESUMO
This report describes cytological, genetic and biochemical studies designed to characterize two γ-radiation induced, apparent "underproducer" variants of the rosy locus (ry:3-52.0), ryps1149 and ryps11136. The following observations provide a compelling basis for their diagnosis as heterochromatic position effect variants. (1) They are associated with rearrangements that place heterochromatin adjacent to the rosy region of chromosome 3 (87D). (2) The effect of these mutations on rosy locus expression is subject to modification by abnormal Y chromosome content. (3) The rearrangement alters only the expression of the rosy allele on the same chromosome (cis-acting). (4) The Y chromosome modification is only on the position-affected allele's expression. (5) The recessive lethality associated with the rearrangements relate to specific rosy region vital loci, and for ryps 11136, the lethality is not Y chromosome modified. (6) The peptide product of the position-affected allele is qualitatively normal by several criteria. (7) Heterozygous deletion of 87E2-F2 is a suppressor of the rosy position effect. (8) The rosy position effect on XDH production may be assayed in whole larvae and larval fat body tissue as well as in adults.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1202427Documentos Relacionados
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