Heterologous mischarging as a means of tRNA fractionation. I. Behaviour of E.coli phenylalanyl-tRNAVal1 on BD-cellulose

AUTOR(ES)

Rosenmann, Eduardo

RESUMO

The chromatographic behaviour of E.coli tRNAVal1, Val-tRNAVal1 and Phe-tRNAVal1 was studied on BD-cellulose columns. At pH 4.0 and 4°C the elution position of Phe-tRNAVal1 was not affected by the presence of absence of Mg2+, whereas Val-tRNAVal1 was slightly retarded when Mg2+ was ommited. It is postulated that the amino acid and its nature influence the structure that the aminoacyl-tRNA assumes. Under suitable conditions the heteroaminoacylated Phe-tRNAVal1 eluted significantly later than other tRNAs. This fact showed that heterologous mischarging can be a useful step in tRNA purification methods.

Documentos Relacionados

• Affinity labeling of phenylalanyl-tRNA synthetase from E.coli MRE-600 by E.coli tRNAphe containing photoreactive group
• Unusual anticodon loop structure found in E.coli lysine tRNA.
• Mercury(II) binding to s4U in E.coli tRNAVal
• Detection of nucleoside Q precursor in methyl-deficient E.coli tRNA.
• Purification of Leucine tRNA Isoaccepting Species from Soybean Cotyledons: I. Benzoylated Diethylamino Cellulose Fractionation, N-Hydroxysuccinimide Modification, and Characterization of Product 1