High Bacterial Diversity in Permanently Cold Marine Sediments
AUTOR(ES)
Ravenschlag, Katrin
FONTE
American Society for Microbiology
RESUMO
A 16S ribosomal DNA (rDNA) clone library from permanently cold marine sediments was established. Screening 353 clones by dot blot hybridization with group-specific oligonucleotide probes suggested a predominance of sequences related to bacteria of the sulfur cycle (43.4% potential sulfate reducers). Within this fraction, the major cluster (19.0%) was affiliated with Desulfotalea sp. and other closely related psychrophilic sulfate reducers isolated from the same habitat. The cloned sequences showed between 93 and 100% similarity to these bacteria. Two additional groups were frequently encountered: 13% of the clones were related to Desulfuromonas palmitatis, and a second group was affiliated with Myxobacteria spp. and Bdellovibrio spp. Many clones (18.1%) belonged to the γ subclass of the class Proteobacteria and were closest to symbiotic or free-living sulfur oxidizers. Probe target groups were further characterized by amplified rDNA restriction analysis to determine diversity within the groups and within the clone library. Rarefaction analysis suggested that the total diversity assessed by 16S rDNA analysis was very high in these permanently cold sediments and was only partially revealed by screening of 353 clones.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=99730Documentos Relacionados
- Prokaryotic Diversity in Zostera noltii-Colonized Marine Sediments
- Statistical Approaches for Estimating Actinobacterial Diversity in Marine Sediments
- Phylogenetic analysis of the bacterial communities in marine sediments.
- Diversity of Thiosulfate-Oxidizing Bacteria from Marine Sediments and Hydrothermal Vents†
- Distribution and Diversity of Archaeal and Bacterial Ammonia Oxidizers in Salt Marsh Sediments▿