High-frequency switching in Candida strains isolated from vaginitis patients.

AUTOR(ES)

Soll, D R

RESUMO

High-frequency switching and strain variability at the site of infection was assessed in 11 patients with acute Candida albicans vaginitis. By cloning cells directly from the site of infection, it was demonstrated that 4 of the 11 isolates contained multiple-switch phenotypes at the site of infection and that 9 of the 11 isolates were in a high-frequency mode of switching (10(-2) to 10(-3)). Isolates could be separated into four general categories of switching repertoires. To demonstrate that multiple phenotypes at the site of a single infection represented the same strain, EcoRI digests of total cell DNA were separated on agarose gels, and Southern hybridization patterns with two cloned midrepeat sequences were compared.

Documentos Relacionados

• High-frequency switching in Candida albicans.
• "White-opaque transition": a second high-frequency switching system in Candida albicans.
• High-frequency leader sequence switching during coronavirus defective interfering RNA replication.
• Phenotypic switching of variable surface lipoproteins in Mycoplasma bovis involves high-frequency chromosomal rearrangements.
• Isolation of High-Frequency Recombining Strains from Escherichia coli Containing the V Colicinogenic Factor