High incidence of type II autoantibodies in pernicious anaemia.
AUTOR(ES)
Waters, H M
RESUMO
AIMS: To investigate the incidence of type II autoantibodies to intrinsic factor in pernicious anaemia. METHODS: Three hundred and forty four serum samples submitted for intrinsic factor antibody (IFAB) analysis on clinical or laboratory grounds were tested by an established radioassay and a new enzyme linked immunosorbent assay (ELISA) method for type I and total IFAB, respectively. Sixty of these were found to be positive by ELISA; this method was used to test further, 40 samples of adequate volume for types I and II antibodies. RESULTS: Type II antibodies were detected in 39 of the 40 sera tested. A comparative analysis indicated that seven samples contained pure type II antibody, being positive for total and type II by ELISA, but negative for type I by both the ELISA and radioassay technique. CONCLUSIONS: The occurrence of type II antibody, both alone and in combination with type I, seems to be more common than has previously been recognised, and emphasises the advantage of using a technique which will detect both types of antibody.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=501112Documentos Relacionados
- Aminoaciduria in pernicious anaemia.
- Rheumatoid arthritis and pernicious anaemia.
- Longitudinal study of circulating gastric antibodies in pernicious anaemia.
- Deficient catecholamine release as the basis of orthostatic hypotension in pernicious anaemia.
- Immunoglobulin and intrinsic factor antibody in the sera of patients with pernicious anaemia.