High Rates of Conjugation in Bacterial Biofilms as Determined by Quantitative In Situ Analysis
AUTOR(ES)
Hausner, Martina
FONTE
American Society for Microbiology
RESUMO
Quantitative in situ determination of conjugative gene transfer in defined bacterial biofilms using automated confocal laser scanning microscopy followed by three-dimensional analysis of cellular biovolumes revealed conjugation rates 1,000-fold higher than those determined by classical plating techniques. Conjugation events were not affected by nutrient concentration alone but were influenced by time and biofilm structure.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=91555Documentos Relacionados
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