High resolution hydroxyl radical footprinting of the binding of mithramycin and related antibiotics to DNA.
AUTOR(ES)
Cons, B M
RESUMO
The preferred binding sites for mithramycin on three different DNA fragments have been determined by hydroxyl radical footprinting. Sequences which appear as one long protected region using DNAase I as a footprinting probe are resolved into several discrete binding domains. Each drug molecule protects three bases from radical attack, though adjacent regions show attenuated cleavage. Mithramycin and the other related compounds induce similar footprinting patterns and appear to recognise GC rich regions with a preference for those containing the dinucleotide step GpG. The ability of each such site to bind the drug depends on the sequence environment in which it is located. The data are consistent with mithramycin binding to the DNA minor groove.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=318169Documentos Relacionados
- Hydroxyl radical "footprinting": high-resolution information about DNA-protein contacts and application to lambda repressor and Cro protein.
- Investigations into the sequence-selective binding of mithramycin and related ligands to DNA.
- Evidence for a conformational change in the DNA gyrase-DNA complex from hydroxyl radical footprinting.
- Hydroxyl radical footprints reveal novel structural features around the NF I binding site in adenovirus DNA.
- Hydroxyl radical "footprinting" of RNA: application to pre-mRNA splicing complexes.