High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae

AUTOR(ES)

Louis, Matthieu

FONTE

MyJove Corporation

RESUMO

Olfactory responses in Drosophila larvae have been traditionally studied in Petri dishes comprising a single peripheral odor source. In this behavioral paradigm, the experimenter usually assumes that the rapid diffusion of odorant molecules from the source leads to the creation of a stable gradient in the dish. To establish a quantitative correlation between sensory inputs and behavioral responses, it is necessary to achieve a more thorough characterization of the odorant stimulus conditions. In this video article, we describe a new method allowing the construction of odorant gradients with stable and controllable geometries. We briefly illustrate how these gradients can be used to screen for olfactory defects (full and partial anosmia) and to study more subtle features of chemotaxis behavior.

Documentos Relacionados

• High-resolution tracking of microtubule motility driven by a single kinesin motor.
• High-resolution computed tomography.
• Mapping of odor-related neuronal activity in the olfactory bulb by high-resolution 2-deoxyglucose autoradiography.
• High-Resolution Shadowing of Transfer RNA
• High-resolution simulations of gravity currents