High similarity of Trypanosoma cruzikDNA genetic profiles detected by LSSP-PCR within family groups in an endemic area of Chagas disease in Brazil

AUTOR(ES)

Alkmim-Oliveira, Sandra Maria, Kappel, Henrique Borges, Andrade, Cristiane Pontes, Prata, Aluízio, Ramirez, Luis Eduardo, Correia, Dalmo, Lages-Silva, Eliane

FONTE

Rev. Soc. Bras. Med. Trop.

DATA DE PUBLICAÇÃO

2014-10

RESUMO

IntroductionDetermining the genetic similarities among Trypanosoma cruzi populations isolated from different hosts and vectors is very important to clarify the epidemiology of Chagas disease.MethodsAn epidemiological study was conducted in a Brazilian endemic area for Chagas disease, including 76 chronic chagasic individuals (96.1% with an indeterminate form; 46.1% with positive hemoculture).ResultsT. cruzi I (TcI) was isolated from one child and TcII was found in the remaining (97.1%) subjects. Low-stringency single-specific-primer-polymerase chain reaction (LSSP-PCR) showed high heterogeneity among TcII populations (46% of shared bands); however, high similarities (80-100%) among pairs of mothers/children, siblings, or cousins were detected.ConclusionsLSSP-PCR showed potential for identifying similar parasite populations among individuals with close kinship in epidemiological studies of Chagas disease.

Documentos Relacionados

• Chagas disease: morbidity profile in an endemic area of Northeastern Brazil
• Analysis of the seroprevalence of and factors associated with Chagas disease in an endemic area in Northeastern Brazil
• Lack of evidence of seronegative infection in an endemic area of Chagas disease
• Predominance of Trypanosoma rangeli infection in children from a Chagas disease endemic area in the west-shore of the Panama canal
• Tuberculin skin testing in inflammatory bowel disease patients from an endemic area of Brazil