Host Specificity of Salmonella typhimurium Deoxyribonucleic Acid Restriction and Modification

AUTOR(ES)

Slocum, Harvey

RESUMO

The restriction and modification genes of Salmonella typhimurium which lie near the thr locus were transferred to a restrictionless mutant of Escherichia coli. These genes were found to be allelic to the E. coli K, B, and A restriction and modification genes. E. coli recombinants with the restriction and modification host specificity of S. typhimurium restricted phage λ that had been modified by each of the seven known host specificities of E. coli at efficiency of plating levels of about 10−2. Phage λ modified with the S. typhimurium host specificity was restricted by six of the seven E. coli host specificities but not by the RII (fi− R-factor controlled) host specificity. It is proposed that the restriction and modification enzymes of this S. typhimurium host specificity have two substrates, one of which is a substrate for the RII host specificity enzymes.

Documentos Relacionados

• Salmonella typhimurium SA host specificity system is based on deoxyribonucleic acid-adenine methylation.
• Transfection of Escherichia coli and Salmonella typhimurium Spheroplasts: Host-Controlled Restriction of Infective Bacteriophage P22 Deoxyribonucleic Acid
• Genetics of Host-Controlled Restriction and Modification of Deoxyribonucleic Acid in Escherichia coli
• Deoxyribonucleic acid restriction and modification systems in Salmonella: chromosomally located systems of different serotypes.
• R Factor-Controlled Restriction and Modification of Deoxyribonucleic Acid: Restriction Mutants