Human aldolase isozyme gene: the structure of multispecies aldolase B mRNAs.
AUTOR(ES)
Sakakibara, M
RESUMO
A complete nucleotide sequence of human aldolase B mRNA was determined with a recombinant cDNA (pHABL120-3). The cDNA insert was composed of 1,652 bases excluding poly(A) tail and the sequence was consistent with the previous results reported by others. However, S1 nuclease mapping and subsequent genomic analysis allowed us to know that the clone possesses two more sites corresponding to 5'-termini in the 5'-noncoding region and another site of polyadenylation in the 3'-noncoding region. In fact, the major aldolase B mRNA species occupying 90% of the total mRNAs initiated at the predominant position corresponding to the position around -82 of the 5'-noncoding sequence in pHABL120-3 and terminated at the distal polyadenylation site. Second species accounting for 9% of the mRNAs initiated at the same site and terminated at the proximal polyadenylation site. The remainings have a longer 5'-noncoding sequence which starts from further upstream region of the major one and pHABL120-3 corresponds to one of these largest clones.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=321849Documentos Relacionados
- The human gastrin/cholecystokinin type B receptor gene: alternative splice donor site in exon 4 generates two variant mRNAs.
- Characterization of the chicken vimentin gene: Single copy gene producing multiple mRNAs
- The human L-myc gene encodes multiple nuclear phosphoproteins from alternatively processed mRNAs.
- Molecular evolution of human and rabbit beta-globin mRNAs.
- Transcriptional analyses of interferon-inducible mRNAs.