Human Antibody Response to Three Meningococcal Outer Membrane Antigens: Comparison by Specific Hemagglutination Assays
AUTOR(ES)
Zollinger, W. D.
RESUMO
Three cell surface antigens, protein, lipopolysaccharide, and polysaccharide, were purified from group B and group C strains of Neisseria meningitidis representing a variety of serotypes. Chemical analysis indicated that cross-contamination was on the order of 1%. Sensitization of sheep erythrocytes with these antigens resulted in highly specific passive hemagglutination assays for the three kinds of antigens. Paired human sera from several groups of individuals were tested by hemagglutination for antibody against each of the antigens. Patients with group B or C systemic meningococcal disease showed increases in antibody titer against all three kinds of antigens, but the antibody response to B polysaccharide was low compared with the response to C polysaccharide. Nasopharyngeal carriers of group B meningococci showed significant increases in titer only against the protein antigens, and noncarriers who received a C-polysaccharide vaccine had a specific response to the C polysaccharide. A given protein or lipopolysaccharide antigen reacted on the average equally well with either group B or C convalescent sera. These results suggest that all three antigens may play a role in the broad human immunity following natural infection.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=423048Documentos Relacionados
- Human antibody responses to meningococcal outer membrane antigens after three doses of the Norwegian group B meningococcal vaccine.
- Human immunoglobulin G subclass immune response to outer membrane antigens in meningococcal group B vaccine.
- Antibody responses to serogroup B meningococcal outer membrane antigens after vaccination and infection.
- Antibody Response to Bacterial Antigens: Characteristics of Antibody Response to Somatic Antigens of Salmonella typhimurium
- Human immune response to meningococcal outer membrane protein epitopes after natural infection or vaccination.