Human basic fibroblast growth factor gene encodes four polypeptides: three initiate translation from non-AUG codons.
AUTOR(ES)
Florkiewicz, R Z
RESUMO
Human basic fibroblast growth factor (bFGF) is an angiogenic polypeptide mitogen present in a wide variety of mesoderm- and neuroectoderm-derived tissues. bFGF cDNA and genomic clones predict a 17.8-kDa (155-amino acid) gene product based on the presence of a single putative translational initiator ATG codon. However, a bFGF protein isolated from human placenta contains two additional amino acids NH2-terminal to the predicted initiator methionine. We report here that the human cell line SK-HEP-1 coexpresses four molecular forms (17.8, 22.5, 23.1, and 24.2 kDa) of bFGF. The 17.8-kDa bFGF protein is translationally initiated at the previously predicted methionine (AUG) codon, whereas the 22.5-, 23.1-, and 24.2-kDa proteins initiate at unusual non-AUG codons. The higher molecular weight forms are colinear NH2-terminal extensions of the 18-kDa bFGF.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=287371Documentos Relacionados
- Human basic fibroblasg growth factor gene encodes four polypeptides: Three initiate translation from non-AUG codons
- Saccharomyces cerevisiae ribosomes recognize non-AUG initiation codons.
- Efficiency of translation initiation by non-AUG codons in Saccharomyces cerevisiae.
- Context effects and inefficient initiation at non-AUG codons in eucaryotic cell-free translation systems.
- Non-AUG Initiation of AGAMOUS mRNA Translation in Arabidopsis thaliana
