Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization.
AUTOR(ES)
Bell, G I
RESUMO
Complementary DNA clones encoding the human kidney epidermal growth factor (EGF) precursor have been isolated and sequenced. They predict the sequence of a 1,207 amino acid protein which contains EGF flanked by polypeptide segments of 970 and 184 residues at its NH2- and COOH-termini, respectively. The structural organization of the human EGF precursor is similar to that previously described for the mouse protein and there is 66% identity between the two sequences. Transfection of COS-7 cells with the human EGF precursor cDNA linked to the SV40 early promoter indicate that it can be synthesized as a membrane protein with its NH2-terminus external to the cell surface. The human EGF precursor gene is approximately 110 kilobase pairs and has 24 exons. Its exon-intron organization revealed that various domains of the EGF precursor are encoded by individual exons. Moreover, 15 of the 24 exons encode protein segments that are homologous to sequences in other proteins. Exon duplication and shuffling appear to have played an important role in determining the present structure of this protein.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=311869Documentos Relacionados
- Gene encoding human growth hormone-releasing factor precursor: structure, sequence, and chromosomal assignment.
- Human T-cell growth factor: partial amino acid sequence, cDNA cloning, and organization and expression in normal and leukemic cells.
- Human thrombopoietin: gene structure, cDNA sequence, expression, and chromosomal localization.
- Expression of human epidermal growth factor precursor cDNA in transfected mouse NIH 3T3 cells.
- The plant ferredoxin precursor: nucleotide sequence of a full length cDNA clone.