Hybridization of Variants of Chloramphenicol Acetyltransferase Specified by fi+ and fi- R Factors
AUTOR(ES)
Shaw, William V.
RESUMO
An fi- type of R factor for transferable chloramphenicol resistance carries the structural gene for a novel species of chloramphenicol acetyltransferase (EC 2.3.1.99). The enzyme associated with the fi- plasmid is distinct from that described for fi+ R factors. The fi+- and fi--related variants of chloramphenicol acetyltransferase were hybridized in vivo and in vitro. Both techniques yielded only a single symmetrical heteromeric species rather than the three (A3B1, A2B2, and A1B3) hybrids predicted from the tetrameric (identical monomers) structure of chloramphenicol acetyltransferase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=389704Documentos Relacionados
- Chloramphenicol Acetyltransferases Specified by fi− R Factors
- Mechanism of Chloramphenicol Resistance in Staphylococci: Characterization and Hybridization of Variants of Chloramphenicol Acetyltransferase
- Temperature-sensitive Chloramphenicol Acetyltransferase from Escherichia coli Carrying Mutant R Factors
- Covert fi− R Factors in fi− R+ Strains of Bacteria
- Episome-mediated Transfer of Drug Resistance in Enterobacteriaceae X. Restriction and Modification of Phages by fi− R Factors
