Hydrophobic Grid-Membrane Filters: New Approach to Microbiological Enumeration
AUTOR(ES)
Sharpe, A. N.
RESUMO
A square grid pattern, printed in hydrophobic material on conventional membrane filters, subdivided the surface into a plurality of areas. This separated colonies from one another and prevented lateral growth, spreading, and confluence. Very high colony-packing densities were achieved (2,500/in2; ca. 1.61 × 105/cm2) reducing the need to dilute samples. Recovery of organisms was better than on conventional filters, particularly at high inoculum levels. At the same time, visibility of normally pale or diffuse colonies was improved, since colonies grew upwards instead of sideways, and counting was facilitated because colonies grew in orderly arrays.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=186690Documentos Relacionados
- Enumeration of High Numbers of Bacteria Using Hydrophobic Grid-Membrane Filters
- Automation of microbial enumeration: development of a disposable hydrophobic grid-membrane filter unit.
- Automated food microbiology: potential for the hydrophobic grid-membrane filter.
- Detection of Listeria monocytogenes by direct colony hybridization on hydrophobic grid-membrane filters by using a chromogen-labeled DNA probe.
- Improved Aerobic Colony Count Technique for Hydrophobic Grid Membrane Filters