Identification and characterization of a beta-globin promoter-binding factor from murine erythroleukemia cells.
AUTOR(ES)
Stuvé, L L
RESUMO
We have identified a DNA-binding activity with specificity for the beta DRE, an evolutionarily conserved transcriptional regulatory element in mammalian adult beta-globin promoters. This binding activity, which we term beta DRf, for beta-globin direct repeat factor, was detected in fractionated nuclear extracts from the murine erythroleukemia cell line and has been partially purified from undifferentiated cells. beta DRf makes symmetric contacts on the two copies of its recognition sequence on both strands and introduces a bend into the DNA helix upon binding. While the factor displays a low binding affinity for the beta DRE in isolation, it binds to the intact beta-globin promoter and DNA fragments containing multiple beta DRE-binding sites with high affinity. A correlation between beta DRf binding affinity and transcriptional activity of beta DRE mutant promoters suggests that this factor stimulates transcription of the beta-globin promoter in vivo.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=359987Documentos Relacionados
- A directly repeated sequence in the beta-globin promoter regulates transcription in murine erythroleukemia cells.
- Activation of human beta-globin genes from nonerythroid cells by fusion with murine erythroleukemia cells.
- DNA sequences involved in transcriptional regulation of the mouse beta-globin promoter in murine erythroleukemia cells.
- High-level beta-globin expression after retroviral transfer of locus activation region-containing human beta-globin gene derivatives into murine erythroleukemia cells.
- Expression of the human beta-globin gene after retroviral transfer into murine erythroleukemia cells and human BFU-E cells.