Identification and characterization of a cellular protein that cross-reacts with the Epstein-Barr virus nuclear antigen.
AUTOR(ES)
Luka, J
RESUMO
A 62,000-dalton (62K) cell protein reacts with antisera to the 72K polypeptide of the Epstein-Barr virus nuclear antigen (EBNA) in immunoblots. This protein was initially detected in EBNA-negative as well as EBNA-positive cell lines with anti-EBNA-positive human sera. A monoclonal antibody raised against the 72K EBNA and an antiserum from a rabbit immunized with the glycine-alanine domain of EBNA also reacted with the cellular protein. The cellular protein was partially purified from Epstein-Barr virus genome-positive and -negative cell lines. Absorption experiments identified a shared antigenic determinant between the 72K EBNA and 62K cellular protein. A comparison of the 62K protein and EBNA by protease digestion did not reveal similar peptides.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=254603Documentos Relacionados
- Characterization of Epstein-Barr virus antigens. I. Biochemical analysis of the complement-fixing soluble antigen and relationship with Epstein-Barr virus-associated nuclear antigen.
- Prediction and demonstration of a novel Epstein-Barr virus nuclear antigen.
- Biochemical characterization of Epstein-Barr virus nuclear antigen 2A.
- Antibodies against a synthetic peptide identify the Epstein-Barr virus-determined nuclear antigen.
- Antibodies against synthetic peptides react with the second Epstein-Barr virus-associated nuclear antigen.