Identification and chromosomal localization of 9 Leishmania (L.) major loci related to resistance against two inhibitors of ergosterol biosynthesis pathway / Identificação e caracterização cromossomal de 9 loci de Leishmania (L.) major relacionados com resistência a inibidores da via de biossíntese do ergosterol

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Ergosterol is an important compound responsible to maintain integrity and fluidity of Leishmania spp. membranes. Starting from an overexpression/selection method, our group has isolated nine different loci of L. (L.) major related to resistance against two inhibitors of the ergosterol biosynthesis pathway, Terbinafine (TBF) and Itraconazole (ITZ). Individual functional analysis of these nine loci in the presence of TBF and/or ITZ (or the ITZ analog Ketoconazole, CTZ), have showed significant levels of resistance after transfection into L. (L.) major wild-type cells, followed by over expression induction. In this work, we show the insert mapping and chromosomal identification of one of these loci (cItz2), as well as discuss the in silico chromosomal analysis of the nine correspondent inserts in the L. (L.) major genome

ASSUNTO(S)

expressão gênica ergosterol/antagonists &inhibitors ergosterol/antagonistas &inibidores gene expression leishmania major drug resistance leishmania major ergosterol/biossíntese ergosterol/biosynthesis resistência a medicamentos

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