Identification and cloning of genes involved in anaerobic sulfite reduction by Salmonella typhimurium.
AUTOR(ES)
Huang, C J
RESUMO
Transposon Tn5 insertions causing anaerobic cysteine auxotrophy were isolated from a Salmonella typhimurium cysI parent (auxotrophic under aerobic but not anaerobic conditions). Insertions in one mutant group appeared to be in cysG. A second group of insertions, designated asr (anaerobic sulfite reduction), were located near map unit 53 on the S. typhimurium chromosome. They did not cause aerobic or anaerobic auxotrophy in a cys1+ background but did prevent dissimilatory sulfite reduction. Plasmids containing asr DNA cloned from wild-type S. typhimurium conferred anaerobic prototrophy and the ability to produce hydrogen sulfide from sulfite on an Escherichia coli cys1 mutant.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=213397Documentos Relacionados
- Characterization of anaerobic sulfite reduction by Salmonella typhimurium and purification of the anaerobically induced sulfite reductase.
- Molecular cloning and expression of the ilvGEDAY genes from Salmonella typhimurium.
- Identification of ancillary fim genes affecting fimA expression in Salmonella typhimurium.
- Identification of the products and nucleotide sequences of two regulatory genes involved in the exogenous induction of phosphoglycerate transport in Salmonella typhimurium.
- Genetic characterization and molecular cloning of the tripeptide permease (tpp) genes of Salmonella typhimurium.