Identification and occurrence of Vibrio cholerae flagellar core proteins in isolated outer membrane.
AUTOR(ES)
Richardson, K
RESUMO
Sodium dodecyl sulfate-urea polyacrylamide gel electrophoresis of outer membranes from a flagellated and an isogenic nonflagellated strain of Vibrio cholerae (classical, Inaba) suggested that two proteins were absent from the nonflagellated strain. Immunoblot examination of such preparations demonstrated that two proteins, present only in outer membrane from the flagellated strain, were associated with flagella. Analysis of purified flagellar cores from strains CA401 and N16961 (El Tor, Inaba) by electron microscopy, sodium dodecyl sulfate-urea polyacrylamide gel electrophoresis, and immunoblotting showed that these two proteins, with apparent molecular weights of 47,000 and 49,000, composed the flagellar core. Antiserum specific for flagellar core proteins did not agglutinate or inhibit the motility of intact V. cholerae. These latter findings suggested that, for intact cells, the flagellar core proteins are not accessible to antibody.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=261354Documentos Relacionados
- Identification and preliminary characterization of Vibrio cholerae outer membrane proteins.
- Identification and Strain Differentiation of Vibrio cholerae by Using Polyclonal Antibodies against Outer Membrane Proteins
- Composition and immunochemical properties of outer membrane proteins of Vibrio cholerae.
- Vibrio cholerae expresses iron-regulated outer membrane proteins in vivo.
- Evaluation of the human immune response to outer membrane proteins of Vibrio cholerae.