Identification of a cis-acting element controlling induction of early gene expression in Dictyostelium discoideum.

AUTOR(ES)

May, T

RESUMO

Using a new promoter analysis transformation vector for Dictyostelium discoideum (PAV-CAT), we have defined cis-acting elements in the promoter of the cyclic AMP-induced early-expressed gene A11H2, which encodes an alpha-fucosidase-related protein (A. Müller-Taubenberger, M. Westphal, A. Noegel, and G. Gerisch, FEBS Lett. 246:185-192, 1989). Sequences responsible for developmentally regulated gene induction could be separated from the basal promoter that conferred low levels of transcriptional activity. By gel shift experiments, we present evidence that the cis-acting element is the target of a trans-acting factor that by itself is subject to developmental regulation.

Documentos Relacionados

• Identification of a cis-acting DNA antisilencer element which modulates vimentin gene expression.
• Pineal expression-promoting element (PIPE), a cis-acting element, directs pineal-specific gene expression in zebrafish
• The neuronal identifier element is a cis-acting positive regulator of gene expression.
• Identification of a cis-Acting Replication Element within the Poliovirus Coding Region
• Delineation of a previously unrecognized cis-acting element required for HLA class II gene expression.